Research

Research Infrastructures

10-litre bioreactors (2 units)


/// Features: - Two 14-litre bioreactors with a 10-litre working volume - Tanks enabling the addition of liquid (base, acid, antifoam, substrate feed) - Possibility to use batch, fed-batch, and continuous processing - Automated control system equipped with the New Brunswick interface and BioCommand software - Scale-up of bioprocesses - Inoculation reactor of the 40-litre bioreactor /// Examples of applications: - Scale-up of aerobic and anaerobic fermentation bioprocesses - Fermentation of large volumes for the purpose of biomass separation, extraction, and biomolecule purification tests - Scale-up of process control automation by computer interface - Scale-up of heterotrophic algae production processes

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 10-litre bioreactors (2 units)

2.4-litre bioreactor (4 units)


/// Features: - A 2.4 L bioreactor with a 2-litre working volume - Tanks enabling the addition of liquid (base, acid, antifoam, substrate feed) - Possibility to use batch, fed-batch, and continuous processing - Automated control system equipped with the New Brunswick interface and BioCommand software - Laboratory scale fermentation bioprocess exploratory studies and development /// Examples of applications: - Aerobic and anaerobic fermentation bioprocess development - Exploratory studies (O2 transfer rate tests, culture media tests) - Optimization of fermentation conditions (O2, ventilation, temperature, pH, agitation, and feed) - Preliminary assessment of bioprocessing needs - Assessment of bioprocess automation needs

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 2.4-litre bioreactor (4 units)

300-litre anaerobic digesters (biomethanizer)


/// Features: - Two 300-litre digesters with an effective working volume of 200 L - Two 300-litre pre-treatment / storage digester feed tanks - Automated 24-hour, continuous digester feed programming - Agitation of the four tanks, connected to the automate, using impeller pumps - Variable feed flow ranging from 5 L/min to 50 L/min - Fully watertight and made of stainless steel - Two-inch long inlet and outlet with valves for input and discharge of digestate - Outlet for gas collection in a Tedlar bag and septum for GC-TCD analysis - GCTCD biogas analysis (N2, O2, CO2, CH4) and FTIR (H2S) - Double wall coil heating, ranging from 21 °C to 60 °C - Possibility to test gas purification membranes at the exits of the two digesters /// Examples of applications: - Pre-pilot phase process development on a continuous basis (fed-batch) - Methane production mechanical parameter optimization (OLR, HRT) - Methane production physical-chemical parameter optimization (pH, T°C, etc.) - Long-term toxicity and inhibition tests relating to certain inputs / co-inputs - Assessment of biogas production using pre-treated inputs (enzymatic/thermal/chemical)

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 300-litre anaerobic digesters (biomethanizer)

4 500-litre anaerobic digester


/// Features: - One 4 500-litre digester with an effective working volume of 4 000 L - One 1 500-litre pre-treatment / storage feed tank - Automated 24-hour, continuous digester feed programming - Agitation of the two tanks, connected to the automate, using a Netschz progressive cavity pump - Variable feed flow ranging from 50 L/min to 500 L/min - Fully watertight and made of stainless steel - Four-inch long inlet and outlet with pneumatic valves for digestate input and discharge - Double wall coil heating, ranging from 21°C to 70°C - Outlet to a flare off burner for gas disposal with flow measurement and gas collection for analysis - Biogas qualitative analysis (O2, N2, CO2, CH4, H2S) using a portable Sewerin Multitec 540 analyzer /// Examples of applications: - Pilot phase process development on a continuous basis (fed-batch) - Methane production mechanical parameter optimization (OLR, HRT) - Methane production physical-chemical parameter optimization (pH, T°C, etc.) - Long-term toxicity and inhibition tests relating to certain inputs / co-inputs - Assessment of pre-treated input biogas production (enzymatic/thermal/chemical)e biogaz avec intrant prétraité (enzymatique/thermique/chimique)

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 4 500-litre anaerobic digester

4-compartment membrane filtration unit


/// Based on 4 Sourirajan-type cells, this unit allows you to: - Characterization of industrial membranes or in situ manufacturing (14 cm diameter coupon) Hyperfiltration, nanofiltration, ultrafiltration type (see characterization method). - Effect of tangential flow. /// Other characteristics: - Mounting material on a rolling trolley (standard door passage). - 4 Sourirajan-type cells. - 10 liter tank Box for micro filtration cartridge. - Piston diagram type pump (1000 Psig, 4gpm) pressure independent of the flow rate. - Temperature, pressure, flow sensor. - Pressure gauge, flowmeter and thermometer. - Pressure control valve. - Safety valve with maximum pressure adjustment. - Data acquisition and control system. - High efficiency variable speed motor. - Electronic speed variator. - Computer and software for control and data acquisition.

Institution
  • Cégep Saint-Laurent
Location
Montréal (Québec)
Contact person
Édith Laflamme, Director, Centre des technologies de l'eau
edlaflamme@cegepsl.qc.ca, 514 747-6521, ext. 8676
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 4-compartment membrane filtration unit

40 L bioreactor with a 30-litre working volume


/// Features: - 40 L bioreactor with a 30-litre working volume - Tanks enabling the addition of liquid (base, acid, antifoam, substrate feed) - Possibility to use batch, fed-batch, and continuous processing - Tank pressurization up to 5 bar, unique in Quebec - Automated control system - Scale-up of bioprocesses /// Exemples d’application: - Scale-up of aerobic and anaerobic fermentation bioprocesses - Scale-up of heterotrophic algae production processes - Study of the improvement of the rate of oxygen transfer by pressurization - Technical and economic studies for the purpose of analyzing bioprocess cost-effectiveness - Metal nanoparticle production by microorganism

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 40 L bioreactor with a 30-litre working volume

7-litre anaerobic digesters


/// Features: - Six 7-litre digesters, with an effective working volume of 5 L - Mechanical blade agitation at a fixed speed of ~ 40 RPM - Fully watertight and made of stainless steel - Two-inch long inlet and outlet with valves for input and discharge - Outlet for gas collection in a Tedlar bag and septum for GC-TCD analysis - GCTCD biogas analysis (N2, O2, CO2, CH4) and FTIR (H2S) - Incubation in a temperature controlled or refrigerated bath ranging from 4 °C to 55 °C /// Examples of applications: - Maintenance of methanogen inoculum for the purpose of performing methane potential tests (BMP) - Continuous laboratory-scale process development (Fed-batch) - Methane production mechanical parameter optimization (OLR, HRT) - Methane production physical-chemical parameter optimization (pH, T°C, etc.) - Long-term toxicity and inhibition tests relating to certain inputs / co-inputs

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 7-litre anaerobic digesters

700-litre bioreactors (500-litre capacity for bioprocess scale-up and a pressure range of up to 5 bar, unique in Quebec)


/// Features: - A 700-litre bioreactor with a 500-litre working volume - Tanks enabling the addition of liquid (base, acid, antifoam, substrate feed) - Possibility to use batch, fed-batch, and continuous processing - Tank pressurization up to 5 bar, unique in Quebec - Automated control system - Scale-up of bioprocesses /// Examples of applications: - Scale-up of aerobic and anaerobic fermentation bioprocesses - Scale-up of heterotrophic algae production processes - Study of the improvement of the rate of oxygen transfer by pressurization - Technical and economic studies for the purpose of analyzing bioprocess cost-effectiveness - Metal nanoparticle production by microorganisms

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 700-litre bioreactors (500-litre capacity for bioprocess scale-up and a pressure range of up to 5 bar, unique in Quebec)

Adaptable earth observation system


The Earth Observation System (EOS) provides the infrastructure needed to study and document ecosystems in the St. Lawrence River Valley (VFSL) in Quebec in order to develop a quantitative predictive model of system-wide interactions in the geobiosphère-climate of the region. A key aspect of EOS is its ability to include a combination of fixed facilities and a flexible mobile infrastructure to observe and analyze the Dynamic Terrestrial System (DES) in remote areas of Quebec. The VFSL climate is characterized by exceptional weather conditions (eg, ice storms), varied uses of land (urban, natural, etc.) and biodiversity flows (species loss and invasion).

Institution
  • McGill University
  • Université du Québec à Montréal (UQÀM)
Location
Montreal (Quebec) and mobile
Contact person
Julie Thériault, Professeur
theriault.julie@uqam.ca, 514 987-3000, poste 4276
Associated axes :
  • Axis Water Availability
    Theme 1: Integrated quantity and quality simulations
  • Axis Water Availability
    Theme 2: Hydrometeorological regime under climate
  • Axis Water Availability
    Theme 3: Flow rates and water levels

Anaerobic chamber (in a nitrogen/CO2 atmosphere)


/// Features: - Capacity to incubate 200 petri dishes on eight individual plates - Inside ambient air temperature between +5 °C and 70 °C, ± 0.25 °C - Continuous AMG gas purge (Anaerobic mixture gas, 5% H2, 20% CO2, 75 % N2) - Palladium catalyst for capturing residual oxygen - Wearing gloves is not required for handling inside the chamber - Lock with vacuum and nitrogen purge cycles to enter and take out material /// Samples: - Petri dishes containing facultative and obligate anaerobic strains - Liquid cultures not requiring stirring - Hypoxia tissue cultures - Oxygen-sensitive material /// Examples of applications: - Anaerobic microbiological tests - Anaerobic strain isolation - Screening tests of microorganisms (wild-type or recombinant); screening tests

Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de Anaerobic chamber (in a nitrogen/CO2 atmosphere)
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