Research

Research Infrastructures

10-litre bioreactors (2 units)


/// Features: - Two 14-litre bioreactors with a 10-litre working volume - Tanks enabling the addition of liquid (base, acid, antifoam, substrate feed) - Possibility to use batch, fed-batch, and continuous processing - Automated control system equipped with the New Brunswick interface and BioCommand software - Scale-up of bioprocesses - Inoculation reactor of the 40-litre bioreactor /// Examples of applications: - Scale-up of aerobic and anaerobic fermentation bioprocesses - Fermentation of large volumes for the purpose of biomass separation, extraction, and biomolecule purification tests - Scale-up of process control automation by computer interface - Scale-up of heterotrophic algae production processes

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 10-litre bioreactors (2 units)

2.4-litre bioreactor (4 units)


/// Features: - A 2.4 L bioreactor with a 2-litre working volume - Tanks enabling the addition of liquid (base, acid, antifoam, substrate feed) - Possibility to use batch, fed-batch, and continuous processing - Automated control system equipped with the New Brunswick interface and BioCommand software - Laboratory scale fermentation bioprocess exploratory studies and development /// Examples of applications: - Aerobic and anaerobic fermentation bioprocess development - Exploratory studies (O2 transfer rate tests, culture media tests) - Optimization of fermentation conditions (O2, ventilation, temperature, pH, agitation, and feed) - Preliminary assessment of bioprocessing needs - Assessment of bioprocess automation needs

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 2.4-litre bioreactor (4 units)

300-litre anaerobic digesters (biomethanizer)


/// Features: - Two 300-litre digesters with an effective working volume of 200 L - Two 300-litre pre-treatment / storage digester feed tanks - Automated 24-hour, continuous digester feed programming - Agitation of the four tanks, connected to the automate, using impeller pumps - Variable feed flow ranging from 5 L/min to 50 L/min - Fully watertight and made of stainless steel - Two-inch long inlet and outlet with valves for input and discharge of digestate - Outlet for gas collection in a Tedlar bag and septum for GC-TCD analysis - GCTCD biogas analysis (N2, O2, CO2, CH4) and FTIR (H2S) - Double wall coil heating, ranging from 21 °C to 60 °C - Possibility to test gas purification membranes at the exits of the two digesters /// Examples of applications: - Pre-pilot phase process development on a continuous basis (fed-batch) - Methane production mechanical parameter optimization (OLR, HRT) - Methane production physical-chemical parameter optimization (pH, T°C, etc.) - Long-term toxicity and inhibition tests relating to certain inputs / co-inputs - Assessment of biogas production using pre-treated inputs (enzymatic/thermal/chemical)

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 300-litre anaerobic digesters (biomethanizer)

4 500-litre anaerobic digester


/// Features: - One 4 500-litre digester with an effective working volume of 4 000 L - One 1 500-litre pre-treatment / storage feed tank - Automated 24-hour, continuous digester feed programming - Agitation of the two tanks, connected to the automate, using a Netschz progressive cavity pump - Variable feed flow ranging from 50 L/min to 500 L/min - Fully watertight and made of stainless steel - Four-inch long inlet and outlet with pneumatic valves for digestate input and discharge - Double wall coil heating, ranging from 21°C to 70°C - Outlet to a flare off burner for gas disposal with flow measurement and gas collection for analysis - Biogas qualitative analysis (O2, N2, CO2, CH4, H2S) using a portable Sewerin Multitec 540 analyzer /// Examples of applications: - Pilot phase process development on a continuous basis (fed-batch) - Methane production mechanical parameter optimization (OLR, HRT) - Methane production physical-chemical parameter optimization (pH, T°C, etc.) - Long-term toxicity and inhibition tests relating to certain inputs / co-inputs - Assessment of pre-treated input biogas production (enzymatic/thermal/chemical)e biogaz avec intrant prétraité (enzymatique/thermique/chimique)

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 4 500-litre anaerobic digester

4-compartment membrane filtration unit


/// Based on 4 Sourirajan-type cells, this unit allows you to: - Characterization of industrial membranes or in situ manufacturing (14 cm diameter coupon) Hyperfiltration, nanofiltration, ultrafiltration type (see characterization method). - Effect of tangential flow. /// Other characteristics: - Mounting material on a rolling trolley (standard door passage). - 4 Sourirajan-type cells. - 10 liter tank Box for micro filtration cartridge. - Piston diagram type pump (1000 Psig, 4gpm) pressure independent of the flow rate. - Temperature, pressure, flow sensor. - Pressure gauge, flowmeter and thermometer. - Pressure control valve. - Safety valve with maximum pressure adjustment. - Data acquisition and control system. - High efficiency variable speed motor. - Electronic speed variator. - Computer and software for control and data acquisition.

Institution
  • Cégep Saint-Laurent
Location
Montréal (Québec)
Contact person
Édith Laflamme, Director, Centre des technologies de l'eau
edlaflamme@cegepsl.qc.ca, 514 747-6521, ext. 8676
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 4-compartment membrane filtration unit

40 L bioreactor with a 30-litre working volume


/// Features: - 40 L bioreactor with a 30-litre working volume - Tanks enabling the addition of liquid (base, acid, antifoam, substrate feed) - Possibility to use batch, fed-batch, and continuous processing - Tank pressurization up to 5 bar, unique in Quebec - Automated control system - Scale-up of bioprocesses /// Exemples d’application: - Scale-up of aerobic and anaerobic fermentation bioprocesses - Scale-up of heterotrophic algae production processes - Study of the improvement of the rate of oxygen transfer by pressurization - Technical and economic studies for the purpose of analyzing bioprocess cost-effectiveness - Metal nanoparticle production by microorganism

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 40 L bioreactor with a 30-litre working volume

7-litre anaerobic digesters


/// Features: - Six 7-litre digesters, with an effective working volume of 5 L - Mechanical blade agitation at a fixed speed of ~ 40 RPM - Fully watertight and made of stainless steel - Two-inch long inlet and outlet with valves for input and discharge - Outlet for gas collection in a Tedlar bag and septum for GC-TCD analysis - GCTCD biogas analysis (N2, O2, CO2, CH4) and FTIR (H2S) - Incubation in a temperature controlled or refrigerated bath ranging from 4 °C to 55 °C /// Examples of applications: - Maintenance of methanogen inoculum for the purpose of performing methane potential tests (BMP) - Continuous laboratory-scale process development (Fed-batch) - Methane production mechanical parameter optimization (OLR, HRT) - Methane production physical-chemical parameter optimization (pH, T°C, etc.) - Long-term toxicity and inhibition tests relating to certain inputs / co-inputs

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 7-litre anaerobic digesters

700-litre bioreactors (500-litre capacity for bioprocess scale-up and a pressure range of up to 5 bar, unique in Quebec)


/// Features: - A 700-litre bioreactor with a 500-litre working volume - Tanks enabling the addition of liquid (base, acid, antifoam, substrate feed) - Possibility to use batch, fed-batch, and continuous processing - Tank pressurization up to 5 bar, unique in Quebec - Automated control system - Scale-up of bioprocesses /// Examples of applications: - Scale-up of aerobic and anaerobic fermentation bioprocesses - Scale-up of heterotrophic algae production processes - Study of the improvement of the rate of oxygen transfer by pressurization - Technical and economic studies for the purpose of analyzing bioprocess cost-effectiveness - Metal nanoparticle production by microorganisms

Institution
  • Cégep de Shawinigan
Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de 700-litre bioreactors (500-litre capacity for bioprocess scale-up and a pressure range of up to 5 bar, unique in Quebec)

Air handling unit stripping and filtration


Pilot for the treatment of industrial wastewater containing volatile compounds, for both pedagogy and research and development. It is a flexible equipment that allows feasibility studies on different types of contaminated water. It is composed of : - 10 l capacity polyethylene feed tank with removable lid - Total drain valve at the bottom of the tank - Raw water supply valve - Variable flow centrifugal feed pump - Supply pipe at the top of the PVC column - Control valve and flowmeter on filter supply - A filtration column on the air stripping bench and modifications on the bench to accept this filtration column

Institution
  • Cégep Saint-Laurent
Location
(mobile) Montréal, Québec
Contact person
Édith Laflamme, Director, Centre des technologies de l'eau
edlaflamme@cegepsl.qc.ca, 514 747-6521, ext. 8676
Associated axes :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
  • Transversal axis: Governance and management
    Theme 2: Risks and disrupters of governance
More info de Air handling unit stripping and filtration

Anaerobic chamber (in a nitrogen/CO2 atmosphere)


/// Features: - Capacity to incubate 200 petri dishes on eight individual plates - Inside ambient air temperature between +5 °C and 70 °C, ± 0.25 °C - Continuous AMG gas purge (Anaerobic mixture gas, 5% H2, 20% CO2, 75 % N2) - Palladium catalyst for capturing residual oxygen - Wearing gloves is not required for handling inside the chamber - Lock with vacuum and nitrogen purge cycles to enter and take out material /// Samples: - Petri dishes containing facultative and obligate anaerobic strains - Liquid cultures not requiring stirring - Hypoxia tissue cultures - Oxygen-sensitive material /// Examples of applications: - Anaerobic microbiological tests - Anaerobic strain isolation - Screening tests of microorganisms (wild-type or recombinant); screening tests

Location
CNETE (Shawinigan)
Contact person
Mohamed Rahni, Chercheur
mrahni@cnete.qc.ca, 819-539-8508
Associated axis :
  • Axis Uses of water
    Theme 1: Water needs and uses of cities
More info de Anaerobic chamber (in a nitrogen/CO2 atmosphere)
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